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of genome integrity. They may be important for DNA replication and maintenance in all organisms. Ligases have been significant reagents in the development of molecular cloning and plenty of subsequent ramifications of DNA biotechnology, including molecular diagnostics and SOLiD sequencing methods. Ligases are elegant and flexible enzymes and therefore are having fun with a research renaissance in light of discoveries that almost all organisms have numerous ligases that both operate in DNA replication (by joining Okazaki fragments) or are committed to unique DNA mend pathways, like nucleotide excision restore, foundation excision repair, one-strand crack restore, or the maintenance of double-strand breaks via nonhomologous finish joining (two–4).

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